Keywords:Chronic Noncommunicable Diseases, Risk Factors, Medical Practitioners, Medical Professionals, Burnout, Trial Participants, Patient Satisfaction, Bronchial Asthma, Community-Acquired Pneumonia

Research groups: Neuroscience in medicine

Research interests:Pulmonology, Evidence based medicine, Public Health, Clinical trials, Transitional research, Medical Statistics, Data Management, Medical Writing, Primary care medicine

Projects performed by the researcher:

• (GC No. 02.512.11.2281) ‘Molecular and genetic patterns of severe asthma’. The federal target program ‘Research and development in priority areas for the development of the scientific and technological complex of Russia for 2007-2012’ (event 1.2. – lineup II)
• (Grant No. 14-04-31752) ‘Study of the microbiotic community of bile associated with opisthorchiasis’, funding period: 2014-2015, funding amount: 800000 RUR, funder: Russian Foundation for Basic Research (RFBR)
• (05.P14.12.0020) ‘The system aims to train qualified specialists in the field of medical research who are proficient in developing integrated clinical trial protocols and conducting medical device clinical trials’/ The federal purpose-oriented program "Development of The Pharmaceutical and Medical Technology Industry of the Russian Federation for the period until 2020 and beyond" (FPP Pharma 2020)
• (18-78-10016) ‘Balancing knowledge reliability and ethical acceptability in clinical trials: from emergence of a randomized controlled trial to precision medicine’ / O.I. Zvonareva, E.A. Borozdina, S.Yu. Martsevich, E.S. Kulikov / Competition for support from the Presidential Research Project Program: Grant in support of research conducted by research groups headed by early career scholars (2018)

Techniques, methods & infrastructure:

• Translational Research
• Clinical trials

Selected publications:

1. Zvonareva, O., Pimenov, I., Kutishenko, N., Mareev, I., Martsevich, S., & Kulikov, E. (2019). Informal professionalization of healthy participants in phase I clinical trials in Russia. Clinical Trials. https://doi.org/10.1177/1740774519877851  (IF 2.257)
2. Saltykova I.V., Ittiprasert W., Nevskaya K.V., Dorofeeva Y.B., Kirillova N.A., Kulikov E.S., Ivanov V.V., Mann V.H., Pershina A.G. and Brindley P.J. (2019) Hemozoin From the Liver Fluke, Opisthorchis felineus, Modulates Dendritic Cell Responses in Bronchial Asthma Patients. Front. Vet. Sci. 6:332. https://doi.org/10.3389/fvets.2019.00332(IF 2.029)
3. Litvinova M., Liu Q., Kulikov Е., Ajelli M. Reactive school closure weakens the network of social interactions and reduces the spread of influenza // PNAS first published June 17, 2019 https://doi.org/10.1073/pnas.1821298116  (IF 9.580)
4. Mareev I., Samykina I., Kolokoltsova M., Pimenov I., Kulikov E., Deev I., Borodulina E., Kobyakova O., Udut V. Informed Consent to Clinical Trial as Factor Determining Participation / Journal of Global Pharma Technology| 2018; 10(08):368-374 (IF 0.18)
5. Kulikov E., Kobyakova O., Deev I., Boykov V., Golubeva Al., Fedosenko S. Assessing the cost-effectiveness of the doctor-patient remotemonitoring system of chronic noncommunicable diseases // European Respiratory Journal 2016 48: PA1031; DOI: 10.1183/13993003.congress-2016.PA1031 (IF 11,807)
6. Kulikov E., Ogorodova L., Deev I., Kirillova N., Fedosenko S. Prevalence of refractory asthma in population with severe asthma // European Respiratory Journal 2016 48: PA4180; http://doi.org/10.1183/13993003.congress2016.PA4180  (IF 11.807)
7. Zvonareva O., Kutishenko N., Kulikov E., Martsevich S. Risks and benefits of trial participation: A qualitative study of participants' perspectives in Russia. Clin Trials. 2015 Dec;12(6):646-53. https://doi.org/10.1177/1740774515589592 Epub 2015 Jun 10. IF 2.715 (IF 2.257)
8. Yuryeva K., Saltykova I., Ogorodova L., Kirillova N., Kulikov E., Korotkaya E., Iakovleva Y., Feoktistov I., Sazonov A., Ryzhov S. Expression of adenosine receptors in monocytes from patients with bronchial asthma. Biochem Biophys Res Commun. 2015 Sep 4;464(4):1314-20. https://doi.org/10.1016/j.bbrc.2015.07.141 Epub 2015 Jul 30. IF 2.466 (IF 2.705)

Keywords: Cholangiocarcinoma, Hepatocellular carcinoma, Opistorchis felineus, Diagnosis, Liver cancer, Epidemiological study, Children, Childhood Diseases, Food allergies, Prevalence

Research groups: Population health under a global threat of trematode infection

Research interests: Infectious Diseases, Pediatrics, Epidemiology, Food Allergy, Allergic Sensitization, Allergy Diagnosis, Public Health, Global Health

Projects performed by the researcher:

1. Development of preclinical diagnostic tools of hepatobiliary tumors associated with Opisthorchis felineus infection in population of endemic regions
2. Epidemiological study of the prevalence of Opisthorchis felineus invasion in the Tomsk region

Selected publications:

1. Golovach E.A., Fedorova O.S., Saprina T.V., Ivanov V.V., Perina E.A., Kovshirina Y.V., Ogorodova L.M. Metabolic syndromes, carbohydrate and lipid metabolism disorders in helminthic infections: review of the literature. Bulletin of Siberian Medicine. 2018;17(4):187-198. (In Russ.) https://doi.org/10.20538/1682-0363-2018-4-187-198
2. Sokolova T.S., Fedorova O.S., Saltykova I.V., Petrov V.A., Fedotova M.M., Kovshirina Y.V., Deev I.A., Ogorodova L.M. Helminths and intestinal microbiota interaction: role in the development of noncommunicable diseases. Bulletin of Siberian Medicine. 2019;18(3):214-225. (In Russ.) https://doi.org/10.20538/1682-0363-2019-3-214-225
3. Kovshirina Y.V., Fedorova O.S., Vtorushin S.V., Kovshirina A.E., Ivanov S.D., Chizhikov A.V., Onishchenko S.V., Ogorodova L.M., Odermatt P. Case report: two cases of cholangiocarcinoma in patients with Opisthorchis felineus infection in Western Siberia, Russian Federation. Am. J. Trop Med. Hyg. 2019; 100 (3): 599-603. https://doi.org/10.4269/ajtmh.18-0652
4. Kovshirina A.E., Fedorova O.S., Kovshirina Y.V., Onishchenko S.V., Taslicki S.S., Katanakhova L.L., Chizhikov A.V., Tataurov I.A., Ororodova L.M. Association between cholangiocarcinoma and liver flukes: review of epidemiological studies. Bulletin of Siberian Medicine. 2020;19(1):150-159. (In Russ.) https://doi.org/10.20538/1682-0363-2020-1-150-159
5. Fedorova O.S., Fedotova M.M., Zvonareva O.I., Mazeina S.V., Kovshirina Y.V., Sokolova T.S., et al. (2020) Opisthorchis felineus infection, risks, and morbidity in rural Western Siberia, Russian Federation. PLoS Negl Trop Dis 14(6): e0008421. https://doi.org/10.1371/journal.pntd.0008421

Keywords: Opisthorchis Felineus, Allergic Disease, Asthma, Food Allergy, Immunology Of Allergic Inflammatory, Genetic Mechanism Of Allergic Diseases

Research groups: Molecular mechanisms of the development of allergic inflammation in endemic helminth foci

Research interests: Immunological and genetic aspects of allergic inflammation against the background of helminth infestation

Grants:

• Fundamental approaches to optimizing the diagnosis and treatment of natural focal invasion of Opisthorchis felineus in endemic regions (Russian Foundation for Fundamental Research)
• Metabolic changes as a reflection of the interaction in the parasite - host system at the level of the target organ and the whole parasite organism (Russian Science Foundation)

Techniques, methods & infrastructure:

• Epidemiological studies
• Cross-sectional studies
• Clinical trials

Selected publications:

1. Vonk JM, Scholtens S, Postma DS, Moffatt MF, Jarvis D, Ogorodova, LM et al. (2017) Adult onset asthma and interaction between genes and active tobacco smoking: The GABRIEL consortium. PLOS ONE 12(3): e0172716. https://doi.org/10.1371/journal.pone.0172716Moffatt MF, Gut IG, Demenais F, et al. A large-scale, consortium-based genomewide association study of asthma. N Engl J Med. 2010;363(13):1211–1221. doi:10.1056/NEJMoa0906312
2. Demenais, F., Margaritte-Jeannin, P., Barnes, K.C., Ludmila M. Ogorodova et al. Multiancestry association study identifies new asthma risk loci that colocalize with immune-cell enhancer marks. Nat Genet 50, 42–53 (2018). https://doi.org/10.1038/s41588-017-0014-7
3. Olga S. Fedorova, Marina M. Fedotova, Tatiana S. Sokolova, Ekaterina A. Golovach, Yulia V. Kovshirina, Tatiana S. Ageeva, Anna E. Kovshirina, Olga S. Kobyakova, Ludmila M. Ogorodova, Peter Odermatt, Opisthorchis felineus infection prevalence in Western Siberia: A review of Russian literature, Acta Tropica, Volume 178, 2018, Pages 196-204, ISSN 0001-706X, https://doi.org/10.1016/j.actatropica.2017.11.018.
4. Gusareva, E.S., Bragina, E.J., Deeva, E.V., Kazakevich, N.V., Puzyrev, V.P., Ogorodova, L.M. and Lipoldová, M. (2006), Cat is a major allergen in patients with asthma from west Siberia, Russia. Allergy, 61: 509-510. doi:10.1111/j.1398-9995.2006.01034.x
5. Demin AM, Pershina AG, Minin AS, Mekhaev AV, Ivanov VV, Lezhava SP, Zakharova AA, Byzov IV, Uimin MA, Krasnov VP, Ogorodova LM. PMIDA-Modified Fe3O4 Magnetic Nanoparticles: Synthesis and Application for Liver MRI. Langmuir. 2018 Mar;34(11) 3449-3458. doi:10.1021/acs.langmuir.7b04023. PMID: 29478322.
6. Ekaterina A. Perina, Vladimir V. Ivanov, Alexandra G. Pershina, Natalya A. Perekucha, Anna N. Dzyuman, Kaminskii I. P., Irina V. Saltykova, Alexey E. Sazonov and Ludmila M. Ogorodova. “Imbalance in the glutathione system in Opisthorchis felineus infected liver promotes hepatic fibrosis.” Acta tropica 192 (2019): 41-48 . https://doi.org/10.1016/j.actatropica.2019.01.017
7. Alexandra G. Pershina, Olga Y. Brikunova, Alexander M. Demin, Oleg B. Shevelev, Ivan A. Razumov, Evgenii L. Zavjalov, Dina Malkeyeva, Elena Kiseleva, Nadezhda V. Krakhmal’, Sergey V. Vtorushin, Vasily L. Yarnykh, Vladimir V. Ivanov, Raisa I. Pleshko, Victor P. Krasnov, Ludmila M. Ogorodova, pH-triggered delivery of magnetic nanoparticles depends on tumor volume, Nanomedicine: Nanotechnology, Biology and Medicine, Volume 23, 2020, 102086, ISSN 1549-9634, https://doi.org/10.1016/j.nano.2019.102086.
8. Yulia Kovshirina, Olga Fedorova, Sergey Vtorushin & Anna Kovshirina & Stanislav Ivanov & Andrey Chizhikov & Sergey Onishchenko & Ludmila Ogorodova & Peter Odermatt. (2018). Case Report: Two Cases of Cholangiocarcinoma in Patients with Opisthorchis felineus Infection in Western Siberia, Russian Federation. The American Journal of Tropical Medicine and Hygiene. https://doi.org/10.4269/ajtmh.18-0652

Keywords: natural products, biological activity of natural compounds, phytochemistry, chromatography, chemical structure elucidation, development of analysis methods

Research groups: "Promising medicines and technologies»

Research interests: research of chemical composition and biological properties of promising plant species as possible sources of new medicines

Techniques, methods & infrastructure:

• biological models using laboratory animals
• extraction, fractionation and chromatographic separation of natural components
• UV, IR, mass-spectrometry, NMR

Selected publications:

1. Avdeeva E., Shults E._,Rybalova T., Reshetov Ya., Porokhova E., Sukhodolo I., Litvinova L., Shupletsova V., Khaziakhmatova O., Khlusov I., Guryev A., Belousov M. Сhelidonic Acid and its Derivatives from Saussurea Controversa: Isolation, Structural elucidation and Influence on the Osteogenic Differentiation of Multipotent Mesenchymal Stromal Cells in Vitro. Biomolecules. 2019; 9(5):189. https://doi.org/10.3390/biom9050189
2. Khlusov I., Avdeeva E., Shupletsova V., Khaziakhmatova O., Litvinova L., Porokhova E., Reshetov Ya., Zvereva I., Mushtovatova L., Karpova M., Guryev A., Sukhodolo I., Belousov M. Comparative In Vitro Evaluation of Antibacterial and Osteogenic Activity of Polysaccharide and Flavonoid Fractions Isolated from the leaves of Saussurea controversa. Molecules. 2019;  24(20):3680. https://doi.org/10.3390/molecules24203680 
3. Avdeeva E., Shults E., Skorokhodova M., Reshetov Ya., Porokhova E., Sukhodolo I., Krasnov E., Belousov M. Flavonol glycosides from Saussurea controversa and their efficiency in experimental osteomyelitis. Planta Medica International Open. 2018; 5:e24-e29. https://doi.org/10.1055/s-0044-100799
4. Avdeeva E.Yu., Skorokhodova M.G.,  Sukhodolo I.V., Porokhova E.D.,  Slizovsky G.V., Mushtovatova L.S., Reshetov Ya.E.,  Ivanov S.D., Belousov M.V. Comparative evaluation of osteogenic activity and the effect on hematopoietic function of bone marrow of fractions of Saussurea controversa and Filipendula ulmaria extracts in experimental osteomyelitis. Bulletin of Siberian medicine. 2019; 18(3):6-15. https://doi.org/10.20538/1682-0363-2019-3-6-14 
5. Avdeeva E.Yu., Skorokhodova M.G., Porokhova E.D.,  Slizovsky G.V., Mushtovatova L.S., Ivanov S.D., Reshetov Ya.E., Belousov M.V. The efficiency of Saussurea controversa and Fillipendula ulmaria extracts on the background of antibiotic therapy of osteomyelitis in experiment. Experimental and clinical pharmacology. 2019; 12:41-46.  https://doi.org/10.30906/0869-2092-2019-82-12-41-46

Keywords: Molecular Pathology, Cytopathology, Human Pathology

Projects performed by the researcher:

• The role of galectins in colorectal cancer: their role in the dysregulation of adaptive immune responses and diagnostic significance.

Selected publications:

1. Kolobovnikova, U.V., Urazova, O.I., Novitsky, V.V. et al. Cytokine-Secreting Activity of Blood Eosinophils in Pulmonary Tuberculosis. Bull Exp Biol Med 153, 319–322 (2012). https://doi.org/10.1007/s10517-012-1705-9
2. Kolobovnikova Yu.V., Urazova O.I., Novitskiy V.V. Peculiarities of The Functional Activity of Blood Eosinophil Granulocytes In Pulmonary Tuberculosis. Bulletin of Siberian Medicine. 2014;13(5):42-48. (In Russ.) https://doi.org/10.20538/1682-0363-2014-5-42-48
3. Kolobovnikova Yu.V., Dmitrieva A.I., Jankovic K.I., Vasilyeva O.A., Purlik I.L., Novitskiy V.V., Urazova O.I., Hardikova S.A. Galectin-1-mediated expression of proteins-regulators of cell cycle and growth factors in gastric cancer. Bulletin of Siberian Medicine. 2017;16(4):165-172. (In Russ.) https://doi.org/10.20538/1682-0363-2017-4-165-172
4. Kolobovnikova Yu.V., Yankovich K.I., Romanova E.V., Dmitrieva A.I., Novitskiy V.V., Urazova O.I. The expression of CCL11/eotaxin, CCR3 receptor and eosinophil peroxidase in tumor tissue in gastric and colon cancers. Bulletin of Siberian Medicine. 2018;17(3):80-87. (In Russ.) https://doi.org/10.20538/1682-0363-2018-3-80-87
5. Kolobovnikova Yu.V., Yankovich K.I., Romanova E.V., Dmitrieva A.I., Urazova O.I., Novitskii V.V., Poletika V.S. VEGF-and EGF-mediated cooperation of eosinophilic granulocytes and tumor cells in gastric and colon cancer. Bulletin of Siberian Medicine. 2019;18(1):211-219. (In Russ.) https://doi.org/10.20538/1682-0363-2019-1-211-219

Keywords: Сomputational Biophysics, Quantitative Methods, Biomedical Instrumentation, Electric, Impedance , Biomedical Imaging

Projects performed by the researcher:

• Development of an algorithm for preclinical diagnosis of equilibrium disorders in multiple sclerosis using a video motion capture system in virtual reality
• Development of a system for diagnosing equilibrium in patients with Parkinson's disease using extremely sensitive systems for detecting movements

Selected publication:

1. Alifirova, V.M., Brazovskii, K.S., Zhukova, I.A. et al. A Method for Quantitative Evaluation of the Results of Postural Tests. Bull Exp Biol Med 161, 439–441 (2016). https://doi.org/10.1007/s10517-016-3433-z
2. Brazovskii, K.S., Pekker, J.S. & Soldatov, A.I. Probe Current Sources for Electrical Impedance Tomography. Biomed Eng 49, 349–353 (2016). https://doi.org/10.1007/s10527-016-9564-8
3. Pekker, Y.S., Tolmachev, I.V., Brazovsky, K.S. et al. Software Package for Assessing Functional Status of Mother and Fetus. Biomed Eng 49, 197–201 (2015). https://doi.org/10.1007/s10527-015-9529-3
4. Brazovskii, K. S., Pekker, J. S., & Umanskii, O. S. (2014). Modelling the Ability of Rheoencephalography to Measure Cerebral Blood Flow, Journal of Electrical Bioimpedance, 5(1), 110-113. doi: https://doi.org/10.5617/jeb.962
5. Demkin, V.P., Pekker, Y.S. & Brazovskii, K.S. Radiation diagnostics: application of supercomputer technologies for three-dimensional visualization of biological objects. Russ Phys J 55, 858–859 (2012). https://doi.org/10.1007/s11182-012-9892-4

Keywords: Tuberculosis, Lymphocytes, Monocytes, Cytokines, Mycobacterium Tuberculosis, Immunology of Infectious Diseases

Research interests: Cell Biology, Physiology of the Blood and Immunity Systems, Hematology

Projects performed by the researcher:

• Molecular factors of dysregulation of homeostasis of immunocompetent blood cells in socially significant diseases. (Council for Grants of the President of the Russian Federation 2018-2020)
• Galectins in colorectal cancer: a role in the dysregulation of adaptive immunity and significance of diagnostics (Council for Grants of the President of the Russian Federation 2019-2020)
• Distant and local mechanisms of generation of various subpopulations of monocytes and their role in the pathogenesis of ischemic cardiomyopathy (Russian Foundation for Basic Research no. 18-015-00160\18, 2018-2020)
• Functional phenotype, molecular mechanisms of differentiation, and cytokine secretory activity of monocytes / macrophages in pulmonary tuberculosis (Russian Foundation for Basic Research no. 19-315-90018\19, 2019-2021)
• The effect of implantation of thin cuticle based on polylactic acid on the morphology of the cornea with bullous keratopathy (Russian Foundation for Basic Research no. 19-415-703005\19, 2019-2020)

Patents:

Churina E.G., Urazova O.I., Novitsky V.V., Fokin V.A. Method for predicting pulmonary tuberculosis with multidrug resistance, No. 2504787 dated 20.01.2014

Churina E.G., Urazova O.I., Novitsky V.V. Method for the diagnosis of secondary immunological insufficiency in pulmonary tuberculosis, No. 2504784 dated 20.01.2014

Chumakova S.P., Shipulin V.M., Urazova OI, Novitsky V.V., Maltseva I.V. Method for predicting moderate and severe hemolysis after coronary artery bypass grafting under extracorporeal circulation, No. 2552925 dated May 14, 2015

Chumakova SP, Urazova OI, Shipulin VM, Novitskiy VV, Petrova IV, Kolobovnikova Yu.V. Method for predicting the degree of hemolytic complication risks after coronary artery bypass grafting under extracorporeal circulation, No. 2542434 dated 21.01.2015.

Selected publications:

1. Chumakova S.P., Shipulin V.M., Urazova O.I et. al. Ischemic cardiomyopathy: blood monocytes and mediators of their differentiation // Annals of the Russian academy of medical sciences. - 2019. - 74 (6) – p. 396-404 doi:10.15690/vramn1185  
2. Kolobovnikova Y.V., Urazova O.I., Vasilieva O.A. et. al. Role of galectins 1 and 3 in the recruitment of eosinophilic granulocytes into tumor tissue in gastric and colon cancers // Annals of the Russian academy of medical sciences. - 2019. - 74 (5) - p. 317-322. doi:10.15690/vramn1189
3. Churina E.G., Sitnikova A.V., Urazova O.I., Chumakova S.P., Vins M.V., Beresneva A.E., Novitskii V.V. Macrophages in bacterial lung diseases: phenotype and functions (review). Bulletin of Siberian Medicine. 2019;18(1):142-154. (In Russ.) https://doi.org/10.20538/1682-0363-2019-1-142-154
4. Chumakova S.P., Vins M.V., Urazova O.I., Azarova D.A., Shipulin V.M., Pryakhin A.S., Bukreeva E.B., Bulanova A.A., Koshel A.P., Churina E.G., Sitnikova A.V., Garganeeva N.P., Novitskii V.V. Subpopulations of blood monocytes in patients with generalized hypoxia. Bulletin of Siberian Medicine. 2019;18(1):277-285. (In Russ.) https://doi.org/10.20538/1682-0363-2019-1-277-285
5. Urazova, O.I., Churina, E.G., Hasanova, R.R., et al., 2019. Association between polymorphisms of cytokine genes and secretion of IL-12p70, IL-18, and IL-27 by dendritic cells in patients with pulmonary tuberculosis. Tuberculosis (Edinb) 115, 56–62. https://doi.org/10.1016/j.tube.2019.02.003
6. Serebryakova, V.A., Urazova, O.I., Novitsky, V.V. et al. Effects of Levofloxacin on Blood Lymphocyte Apoptosis in Patients with Pulmonary Tuberculosis: an In Vitro Study. Bull Exp Biol Med 168, 109–112 (2019). https://doi.org/10.1007/s10517-019-04659-x
7. Urazova O.I., Churina E.G., Kolobovnikova Yu.V., Novitskiy V.V., Karaulov A.V., Nikulina E.L., Poletika V.S. Features of immunoregulation in patients with pulmonary tuberculosis with blood eosinophilia. Bulletin of Siberian Medicine. 2018;17(3):168-179. (In Russ.) https://doi.org/10.20538/1682-0363-2018-3-168-179

Keywords: Oxidative Stress, Reactive Oxygen Species, Oxidative Stress Biomarkers, Free Radicals, Antioxidant Activity, SOD, Biomarkers, Inflammatory Biomarkers, Cell Culture, Antioxidants

Projects performed by the researcher:

• The Role of the Glutathione System in Oxidative Modification of Proteins and Dysregulation of Apoptosis
• The role of heat shock proteins 27 and 70 in redox-dependent regulation of apoptosis

Selected publications:

1. Shakhristova, E.V., Stepovaya, E.A., Rudikov, E.V. et al. Oxidative Modification of Redox Proteins: Role in the Regulation of HBL-100 Cell Proliferation. Bull Exp Biol Med 167, 30–34 (2019). https://doi.org/10.1007/s10517-019-04453-9
2. Shakhristova E.V., Stepovaya E.A., Nosareva O.L., Litvinova L.S., Skuratovskaya D.A., Rudikov E.V., Sadykova A.A., Novitsky V.V. A multi-centre study on the role of the thioredoxin system in breast cancer cell proliferation. Bulletin of Siberian Medicine. 2018;17(4):180-186. (In Russ.) https://doi.org/10.20538/1682-0363-2018-4-180-186
3. Shakhristova E.V., Stepovaya E.A., Sadykova A.A., Novitsky V.V. Protein carbonylation as a possible way to modulate breast cancer cell proliferation. Siberian journal of oncology. 2018;17(6):78-83. (In Russ.) https://doi.org/10.21294/1814-4861-2018-17-6-78-83
4. Shakhristova E.V., Stepovaya E.A., Rudikov E.V., et. al., The role of redox proteins in arresting proliferation of breast epithelial cells under oxidative stress // Annals of the Russian Academy of Medical sciences. - 2018. – 73 (5) - p 289-293. doi:10.15690/vramn1030
5. Stepovaya, E. A., Shakhristova, E. V., Nosareva, O. L., Rudikov, E. V., Egorova, M. Y., Egorova, D. Y., Novitsky, V. V. (2017). Redox-dependent mechanisms of regulation of breast epithelial cell proliferation. Biomeditsinskaya khimiya, 63(2), 159-164. DOI: 10.18097/PBMC20176302159

Keywords:Genetics of Common and Rare Diseases, Cardiovascular Diseases, Atherosclerosis, Molecular Genetics

Grants:

2012-2013, “Phenomenon of paradominant inheritance of complex diseases” (Federal Target Programme “Scientific and Scientific-Pedagogical Personnel of the Innovative Russia” for 2009-2013, contract 8062)

2014-2015, “Epimutations, paradominant inheritance and atherogenesis” (President Grants for Government Support of the Leading Scientific Schools of the Russian Federation, contract NSh-5096.2014.4)

2014-2016, “Postzygotic variability of nuclear and mitochondrial genome during atherosclerotic vascular lesions in men” (Russian Science Foundation, contract №14-15-00305)

2016-2018, “Comparative analysis of the genetic basis of fibrogenesis in several human pathological conditions” (RFBR 16-04-00840)

2016-2018, “Epigenetic mechanisms for regulation of microRNA gene activity in manifest atherosclerosis in human” (Russian Science Foundation, contract № 16-15-10150)

Selected Publications:

1. Nazarenko M.S., Markov A.V., Lebedev I.N., Sleptsov A.A., Frolov A.V., Barbash O.L., Puzyrev V.P. [DNA methylation profiling of the vascular tissues in the setting of       atherosclerosis]. Mol Biol (Mosk). 2013 May-Jun;47(3):398-404. Russian. http://link.springer.com/article/10.1134%2FS0026893313030084
2. Sleptsov A.A., Nazarenko M.S., Lebedev I.N., Skryabin N.A., Frolov A.V., Popov V.A., Barbarash O.L., Barbarash L.S., Puzyrev V.P. [Somatic genome variations in vascular tissues and peripheral blood leukocytes in patients with atherosclerosis]. Genetika. 2014. Aug;50(8):986-95. Russian. https://link.springer.com/article/10.1134/S1022795414080080
3. Nazarenko M.S., Markov A.V., Lebedev I.N., Freidin M.B., Sleptcov A.A., Koroleva I.A., Frolov A.V., Popov V.A., Barbarash O.L., Puzyrev V.P. A comparison of genome-wide DNA methylation patterns between different vascular tissues from patients with coronary heart disease. PLoS One. 2015 Apr 9;10(4):e0122601. doi:10.1371/journal.pone.0122601. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4391864/
4. Golubenko M.V., Nazarenko M.S., Frolov A.V., Sleptsov A.A., Markov A.V., Glushkova M.E., Barbarash O.L., Puzyrev V.P. [Analysis of Heteroplasmy in the Major Noncoding Region of Mitochondrial DNA in the Blood and Atherosclerotic Plaques of Carotid Arteries]. Genetika. 2016 Apr;52(4):497-502. Russian. https://link.springer.com/article/10.1134/S1022795416040049
5. Kucher A.N., Nazarenko M.S., Markov A.V., Koroleva I.A., Barbarash O.L. Variability of Methylation Profiles of CpG Sites in microRNA Genes in Leukocytes and Vascular Tissues of Patients with Atherosclerosis. Biochemistry (Mosc). 2017 Jun;82(6):698-706. DOI: 10.1134/S0006297917060062. http://protein.bio.msu.ru/biokhimiya/contents/v82/full/82060923.html
6. Nazarenko M.S., Sleptcov A.A., Lebedev I.N., Skryabin N.A., Markov A.V., Golubenko M.V., Koroleva I.A., Kazancev A.N., Barbarash O.L., Puzyrev V.P. Genomic structural variations for cardiovascular and metabolic comorbidity. Sci Rep. 2017 Jan 25;7:41268.doi:10.1038/srep41268. https://www.nature.com/articles/srep41268
7. Koroleva I.A., Nazarenko M.S., Kucher A.N. Role of microRNA in Development of Instability of Atherosclerotic Plaques. Biochemistry (Mosc). 2017 Nov;82(11):1380-1390. doi: 10.1134/S0006297917110165. https://link.springer.com/article/10.1134%2FS0006297917110165
8. Nazarenko M.S., Markov A.V., Sleptcov A.A. et al. Comparative Analysis of Gene Expression in Vascular Cells of Patients with Advanced Atherosclerosis. Biochem. Moscow Suppl. Ser. B 13, 74–80 (2019). https://doi.org/10.1134/S1990750819010104 https://link.springer.com/article/10.1134/S1990750819010104
9. Ponomarenko M., Rasskazov D., Chadaeva I., Sharypova E., Drachkova I., Oshchepkov D., Ponomarenko P., Savinkova L., Oshchepkova E., Nazarenko M., Kolchanov N. Candidate SNP Markers of Atherogenesis Significantly Shifting the Affinity of TATA-Binding Protein for Human Gene Promoters show stabilizing Natural Selection as a Sum of Neutral Drift Accelerating Atherogenesis and Directional Natural Selection Slowing It. Int J Mol Sci. 2020 Feb 5;21(3). pii: E1045. doi:10.3390/ijms21031045. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037642/

Keywords: Intraocular Proliferative Syndrome, Eye Diseases, Reparative Regeneration, Cell Technologies, Cytokine Therapy

Research groups: The Biocompatible Materials and Bioengineering

Grants:

• Development of technology for the treatment of endothelial-epithelial dystrophy of the cornea of the human eye with the use of autocytokines (Russian Foundation for promoting innovation, Performer)
• Peripheral uveitis and its complications: clinical and pathogenetic patterns of development, principles of diagnosis and treatment (Council for grants of the President of the Russian Federation to support young Russian scientists, Performer)

Techniques, methods & infrastructure:

• Experimental research
• Clinical trials

Selected publications:

1. Filippova E.O., Krivosheina O.I.Manifestations of experimental bullous keratopathy and the effectiveness of cell therapy. Pathological Physiology and Experimental Therapy. 2020; 64(1):23-30. (In Russ.)https://doi.org: 10.25557/0031-2991.2020.01.23-30
2. Zhigalskaya T.A., Krivosheina O.I., Dzyman A.N., Khlusov I.A.Pathomorphological features of conjunctival and scleral regeneration associated with intraoperative application of Cyclosporin A. Bulletin of Siberian Medicine. 2019;18(3):46-52. (In Russ.) https://doi.org: 10.20538/1682-0363-2019-3-46–52
3. Filippova E.O., Krivosheina O.I. The formation of eyeball musculoskeletal stump using a Ni-Ti implant in vivo. IOP Conference Series Materials Science and Engineering. 2019; 597:012065. https://doi.org:10.1088/1757-899X/597/1/012065
4. KrylovaA.A., Krivosheina O.I. Surgical decompression for endocrine ophthalmopathy: state-of-the-art. Clinical ophthalmology 2019;19(3):154-158. (In Russ.) https://doi.org: 10.32364/2311-7729-2019-19-3-154-158
5. Zhigalskaya T.A., Krivosheina O.I. Clinically approved treatments for refractory glaucoma. Clinical ophthalmology 2019;19(2):110-115. (In Russ.) https://doi.org: 10.32364/2311-7729-2019-19-2-110-115

Keywords: Biomolecules, Phenolic Compounds, Phytochemicals, Bioactivity, Phytochemical Analysis, Natural Product Pharmacology

Research groups: Perspective Drugs and Technologies

Research interests: Natural Compounds Chemistry and Pharmacology

Grants:

• Development of targeted molecules based on frame proteins for the diagnosis and treatment of malignant neoplasms: theranostic approach (Ministry of Education), Performer
• Bionanomaterials and compositions with antimicrobial and wound healing properties based on humic substances (Russian scientific foundation), Supervisor

Techniques, methods & infrastructure:

• Drug development
• Preclinical trials
• Pharmaceutical analysis

Selected publications:

1. Khlusov I., Avdeeva E., Shupletsova V., Khaziakhmatova O., Litvinova L., Porokhova E., Reshetov Y., Zvereva I., Mushtovatova L., Karpova M., Guryev A., Sukhodolo I., Belousov M. Comparative In Vitro Evaluation of Antibacterial and Osteogenic Activity of Polysaccharide and Flavonoid Fractions Isolated from the leaves of Saussurea controversa / Molecules. 2019. 24(20), 3680; https://doi.org/10.3390/molecules24203680
2. Avdeeva E., Shults E., Rybalova T., Reshetov Ya., Porokhova E., Sukhodolo I., Litvinova L., Shupletsova V., Khaziakhmatova O., Khlusov I., Guryev A., Belousov M. Сhelidonic Acid and its Derivatives from Saussurea Controversa: Isolation, Structural elucidation and Influence on the Osteogenic Differentiation of Multipotent Mesenchymal Stromal Cells in Vitro. Biomolecules. 2019, 9(5), 189; https://doi.org/10.3390/biom9050189
3. Podrezova, E. V., Lar’kina, M. S., Yusubov, M. S., Belousov, M. V., Zamanova, M. K., Krivoshchekov, S. V. Validation of a GC-MS Method for Quantitative Determination of Cyclohexanone by Oxidative Cleavage //Pharmaceutical Chemistry Journal. – 2019. – Т. 52. – №. 12. – С. 1021-1025. https://doi.org/10.1007/s11094-019-01945-w
4. Zykova, M. V. Schepetkin, I. A., Belousov, M. V., Krivoshchekov, S. V., Logvinova, L. A., Bratishko, K. A., Yusubov, M.S., Roanenko, S.V., Quinn, M.T. Physicochemical characterization and antioxidant activity of humic acids isolated from peat of various origins //Molecules. – 2018. – Vol. 23. – №. 4. – P. 753. doi:10.3390/molecules23040753
5. Podrezova, E. V., Larkina, M. S., Belousov, M. V., Kirschning, A., Zhdankin, V. V., Yusubov, M.S. Expedient synthesis of long-chain ω-substituted fatty acids and esters from cyclic ketones using iodine and hydrogen peroxide //Synthesis. – 2018. – Т. 50. – №. 20. – P. 4081-4088. DOI: 10.1055/s-0036-1591598

Keywords: Cardiovascular Disease, Metabolic Syndrome, Diabetes, Alpha1- Proteinase Inhibitor, Alpha2-Macroglobuline, Elastase, Trypsin, Chronic Obstructive Pulmonary Disease

Research groups: Proteinase System and Inhibitors in Normal and Pathological Conditions

Research interests: Molecular-Biochemical Diagnosis and Prognosis of Human Diseases

Grants:

The study of volume-dependent mechanisms of regulation of the contractile function of smooth muscle cells of the vessels of the pulmonary circulation (Russian Foundation for Fundamental Research)

Techniques, methods & infrastructure:

• Translational Research
• Clinical trials

Selected publications:

1. Adipocytes directly affect coronary artery disease pathogenesis via induction of adipokine and cytokine imbalances / O Gruzdeva , E. Uchasova, Y. Dyleva, D. Borodkina,O. Akbasheva at al // Frontiers in Immunolog.- 2019 - Volume 10 - Article 2163- p 1-10  Doi: 10.3389/fimmu.2019.02163. eCollection 2019.
2. Relationship between epicardial and perivascular fatty tissue and adipokine-cytokine level in coronary artery disease patients / Gruzdeva O., Uchasovа E., Dyleva Y., Borodkina D., Akbasheva O. at al.// PLOS ONE .- June 7, 2019 p 1-15. Doi: 10.1371/journal.pone.0208156. eCollection 2019.
3. Early Effects of Treatment Low-Dose Atorvastatin on Markers of Insulin Resistance and Inflammation in Patients with Myocardial Infarction /Gruzdeva O., Uchasova E., Dyleva Yu., Akbasheva O., Karetnikova V. and Barbarash O. //Frontiers in Pharmacolog.- 2016. Т. 7. № SEP. С. 324-331 .https://doi.org/10.3389/fphar.2016.00324
4. Effect of different doses of statins on the development of type 2 diabetes mellitus in patients with myocardial infarction /Gruzdeva O., Uchasova E., Dyleva Y., Karetnikova V., Shilov A., Barbarash O., Akbasheva O. // Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy. – 2017. – Т. 10. – С. 481-489. https://doi.org/10.2147/DMSO.S149463
5. The role of adipose tissue and adipokines in the manifestation of type 2 diabetes in the long-term period following myocardial infarction /Barbarash O., Gruzdeva O., Uchasova E., Dyleva Y., Belik E., Akbasheva O., Karetnikova V., Kokov A. // Diabetology and Metabolic Syndrome. – 2016. – Т. 8, № 1. – С. 24-32. Doi: 10.1186/s13098-016-0136-6. eCollection 2016.

Keywords: Drug Development, Encapsulation, Carbohydrate Chemistry and Pharmacology, Polysaccharides, Microencapsulation

Research groups: Anticancer activity of plant polysaccharides, Effect of plant polysaccharides on immune system functions

Research interests: Drug Development, Microencapsulation

Techniques, methods & infrastructure:

• Drug Formulation Development,
• Microencapsulation
• Preclinical investigation
• Carbohydrate Chemistry and Pharmacology

Selected publications:

1. E. Avdeeva, E. Shults, T. Rybalova, Y. Reshetov, E. Porokhova, I. Sukhodolo, L. Litvinova, V. Shupletsova, O. Khaziakhmatova, I. Khlusov, A. Guryev and M. Belousov. Chelidonic Acid and Its Derivatives from Saussurea Controversa: Isolation, Structural Elucidation and Influence on the Osteogenic Differentiation of Multipotent Mesenchymal Stromal Cells In Vitro. Biomolecules 2019, 9(5), 189; doi:10.3390/biom9050189.
2. Igor Khlusov, E. Avdeeva, V. Shupletsova, O. Khaziakhmatova, L. Litvinova, E. Porokhova, Y. Reshetov, I. Zvereva, L. Mushtovatova, M. Karpova, A. Guryev, I. Sukhodolo and M. Belousov. Comparative In Vitro Evaluation of Antibacterial and Osteogenic Activity of Polysaccharide and Flavonoid Fractions Isolated from the leaves of Saussurea controversa. Molecules 2019, 24(20), 3680; doi:10.3390/molecules24203680.
3. Di Martino A., Vlasov S.S., Gur'ev, A.M., Yusubov M.S., Postnikov P.S., Belousov M.V. Development and properties of a new doxorubicin carrier based on surface-modified iron zero-valent microparticles with high encapsulation efficiency and the possibility of its controlled release. Bulletin of Siberian Medicine. 2019; 18 (2): 69–79. DOI: 10.20538/1682-0363-2019-2-69-79.
4. Safonova E.A.,  Lopatina K.A.,  Razina T.G.,  Zueva, E.P., Sadrikina, L.A.,   Gur'ev, A.M.,  Belousov M.V. Correction of Damaging Effects of Cisplatin-Containing Polychemotherapy on the Intestinal Epithelium with Tussilago farfara L. Polysaccharides.  Bulletin of Experimental Biology and Medicine. – V.167, №5. – 2019. – P.616-620. DOI: 10.1007/s10517-019-04582-1.
5. N.V.Belska M.G.Danilets E.S.Trophimova E.G.Uchasova A.A.Ligatcheva, M.V.Belousov V.I.Agaphonov, V.G.Golovchenko M.S.Yusubov Y.P.Belsky Water-soluble polysaccharide obtained from Acorus calamus L. classically activates macrophages and stimulates Th1 response. International Immunopharmacology. - 2010. - V.10. - I. 8.- P. 933-942. DOI: 10.1016/j.intimp.2010.05.005

Keywords: Opisthorchosis, Opisthorchis Felineus, Centaurea Species, Sesquiterpene Lactones, Antiopisthorchiasis Activity, Pharmacological Studies, In Vivo, In Vitro, Standardization, Drug, Phytochemistry

Research groups: Prospective drugs

Research interests: Phytochemistry, Phytotherapy

Grants:

• Preclinical studies of a drug acting on Opisthorchis felineus glutathione-S-transferase for the treatment of opisthorchiasis, (The development of the pharmaceutical and medical industries for 2013–2020, Performer)

Techniques, methods & infrastructure:

• Drug isolation
• Preclinical trials

Selected publications:

1. Perina E.A., Ivanov V.V., Pershina A.G., Perekucha N.A., Dzyuman A.N., Kaminskii I.P., Saltykova I.V., Sazonov A.E., Ogorodova L.M. Imbalance in the glutathione system in Opisthorchis felineus infected liver promotes hepatic fibrosis. Acta Tropica. 2019. Vol. 192. pp. 41-48. https://doi.org/10.1016/j.actatropica.2019.01.017
2. Kaminskii I.P., Lozhnikova A.V., Fomin G.G., Chikov M.V. Biodegradable Materials Application: Experience of the Russian Technological Platform “Medicine of the Future”. Key Engineering Materials. 2016. Vol. 683. pp 440-446. https://doi.org/10.4028/www.scientific.net/KEM.683.440
3. Lezhava S.P., Pershina A.G., Kokova D.A, Perina E.A., Kaminskii I.P., Sazonov A.E., Ogorodova L.M. Opisthorchis felineus Infection and Harmful Metals Bio-concentration: a Pilot Study in Hamster model. Advanced Materials Research. 2015. Vol. 1085. pp 370-375. https://doi.org/10.4028/www.scientific.net/AMR.1085.370
4. Kaminskii I.P., Krasnov E.A., Kadyrova T.V. et al. Quantitative HPLC determination of cynaropicrin in Centaurea Scabiosa dry extract. Pharmaceutical Chemistry Journal. 2011. Vol. 45. pp 560–563. https://doi.org/10.1007/s11094-011-0679-7

Keywords: Depressive Disorders, Schizophrenia, Suicidal Behavior, Addiction, Biopsychosocial Model, Biomedicine, Psychotherapy

Research groups: The influence of institutional factors on mortality from cardiovascular disease and suicide

Research interests: Health Systems Research, Anthropological Psychiatry

Grants:

• Mechanisms of intrapsychic conflict in patients with hypertension (Russian Foundation for Fundamental Research)
• Structuring of data and knowledge, the creation of the intellectual system biopsychosocial rapid diagnostic and prevention of depression in women in the reproductive period (Russian Humanitarian Science Foundation)

Techniques, methods & infrastructure:

• Translational Research
• Clinical trials

Selected publications:

1. Kornetova E.G., Kornetov A.N., Mednova I.A., Dubrovskaya V.V., Boiko A.S., Bokhan N.A., Loonen A.J.M., Ivanova S.A. Changes in body fat and related biochemical parameters associated with atypical antipsychotic drug treatment in schizophrenia patients with or without metabolic syndrome. Frontiers in psychiatry. 2019;10:803. https://doi.org/10.3389/fpsyt.2019.00803
2. Kornetov A.N., Kornetova E.G., Golenkova A.V., Kozlova S.M., Arzhanik M.B., Samoylenko Z.A., Boiko A.S., Semke A.V. Neurocognitive deficits in clinical polymorphism of schizophrenia: typology, expression and syndromal overlaps. Bulletin of Siberian Medicine. 2019;18(2):107-118. (In Russ.) https://doi.org/10.20538/1682-0363-2019-2-107-118
3. Kornetova E.G., Koval S.D., Kornetov A.N., Parshukova D.A., Ivanova S.A., Semke A.V., Gusakova S.V., Bokhan N.A. Brain pathology in schizophrenia: association with clinical and constitutional factors. Yakut Medical Journal. 2019;1:16-20. https://doi.org/10.25789/YMJ.2019.65.05
4. Kornetova E.G., Dmitrieva Е.G., Dubrovskaya V.V., Мednova I.А., Goncharova A.A., Kornetov A.N., Semke A.V., Ivanova S.A., Bokhan N.A. Suicidal behavior in patients with schizophrenia with metabolic syndrome. Suicidology. 2019;10(2):92-98. (In Russ) https://doi.org/10.32878/suiciderus.19-10-02(35)-92-98
5. Yankovskaya A.E., Kornetov A.N., Il'inskikh N.N., Obukhovskaya V.B. An expansion of intelligent systems complex for express-diagnostics and prevention of organizational stress, depression, and deviant behavior on the basis of the biopsychosocial approach. Pattern Recognition and Image Analysis (Advances in Mathematical Theory and Applications). 2017;27(4):783-788. https://doi.org/10.1134/S1054661817040204

Keywords: Pediatric Neurology, Epileptology, Suicidal Behavior, Childhood Behavioral Neurology, Cerebral Palsy, Botulinum Therapy

Research groups: The influence of institutional factors on mortality from cardiovascular disease and suicide

Research interests: Childhood Epilepsy, Cerebral Palsy

Techniques, methods & infrastructure:

• Translational Research
• Clinical trials

Selected publications:

1. Kraeva L.S., Vtorushin S.V., Kuzmina A.V., Kozyritskaya D.V. The possibility of using skin biopsy in the diagnosis of Lafora disease. Bulletin of Siberian Medicine. 2019;18(4):239-243. (In Russ.) https://doi.org/10.20538/1682-0363-2019-4-239-243
2. Kraeva L.S., Koroleva E.S., Alifirova V.M., Kuzmina A.V. A clinical case of neuronal ceroid lipofuscinosis type 2. Bulletin of Siberian Medicine. 2019;18(4):244-248. (In Russ.) https://doi.org/10.20538/1682-0363-2019-4-244-248
3. Krayeva L.S., Alifirova V.M. Clinical and epidemiological characteristic of epilepsy in children and teenagers in Tomsk Region. Bulletin of Siberian Medicine. 2010;9(4):73-76. (In Russ.) https://doi.org/10.20538/1682-0363-2010-4-73-76

Keywords: Genetics, Hereditary Diseases, Population Genetics, Atherosclerosis, Ethnogenomics

Research interests:

• Basic research on the genetic diversity of human populations and the structural and functional organization of the human genome in normal and pathological conditions.
• Genetics of widespread diseases: medical pathogenetics of chronic non-infectious diseases (coronary atherosclerosis, arterial hypertension, bronchial asthma, chronic obstructive pulmonary disease, diabetes mellitus, multiple sclerosis, gestosis), as well as infectious diseases (tuberculosis, tick-borne encephalitis, viral hepatitis).

Selected publications:

1. Puzyrev V.P. Genome liberty and medical pathogenetics // Bulletin of Siberian Medicine, 2002. No. 2. P.16-27.
2. Lebedev I. N., Puzyrev V. P. Epigenetic perspectives of safety in assisted reproductive technologies. // Russian Journal of Genetics, 2007.- V. 43.- No.  9.- P.1157-1171.
3. Puzyrev V. P., Maximova N. P. Hereditary diseases among Yakuts // Russian Journal of Genetics, 2008.- V. 44.- No. 10.- P. 11141-1147.
4. Puzyrev P.Valery, Makeeva A. Oksana, Freidin B. Mxsim. Syntropy, genetic testing and personalized medicine. // Personslized Medicine, 2010.- 7(4).- P.399-405.
5. Puzyrev V. P. Phenome-genome relations and pathogenetics of multifactorial diseases. // Herald of the Russian Academy of Sciences, 2011.- No.  9.- P.17-27.
6. Puzyrev V. P. Multifactorial diseases. // Hereditary Diseases: National Guidelines / Ed. By N. P. Bochkov, E. K. Ginter, V. P. Puzyrev / М.: 2012.- P. 610-660.
7. Nazarenko L. P., Puzyrev V. P. Hereditary diseases in Siberian populations. // In: “Genomics and heals in the developing world” Oxford monographs on medical genetics Chapter 112. “Hereditary diseases among the Yakuts» / Ed. By D. Kumar. Oxford University Press, Inc. Oxford, New York. 2012. P. 1323-1354.
8. Puzyrev V. P. Genetic bases of human comorbidity. // Russian Journal of Genetics, 2015.- V. 51.- No.  4.- P. 408-417.
9. Golubenko M. V., Nazarenko M. C., Frolov A. V., Sleptcov A. A., Marcov A. V., Glushkova M. E., Barbarash O. L., Puzyrev V. P. Heteroplasmia analysis in the major non-coding region of mitochondrial DNA in the blood and atherosclerotic plaques of the carotid arteries. // Russian Journal of Genetics, 2016.- V. 52. No.  4.- P. 497-502.

Keywords: Cardiology, Acute Coronary Syndrome, Myocardial Infarction, Heart Failure, Myocardial Revascularization, Heart Remodelling, Innate Immunity

Research interests: Cardiology and Angiology, Acute Coronary Syndrome, Heart Failure, Cardiovascular Visualization, Immunocompetent Cells and Organs, Regulation of The Immune Response, Internal Illnesses, Clinical Pharmacology, Regenerative Medicine

Grants:

• The development of approach to treatment of myocardial infarction based on autologous bone marrow mononuclear stem cells, (Russian Foundation for Fundamental Research)
• The development of the method of cell therapy for myocardial infarction based on intracoronary transplantation of the mesenchymal stem cells, (Russian Foundation for Fundamental Research)
• The use of stem and progenitor cells from human somatic tissues for reparative therapy and tissue regeneration, (the Federal Target Program government contract)
• Significance of the stiffness of the great arteries and clinical and echocardiography aspects of chronic heart failure in patients with preserved left ventricular ejection fraction after myocardial infarction, (the Grant of the President of the Russian Federation)
• Significance of functional plasticity of monocytes/macrophages in the processes of reparative regeneration and postinfarction cardiac remodeling, (Russian Foundation for Fundamental Research)

Techniques, methods & infrastructure:

• Translational Research
• Clinical trials

Selected publications:

1. Ryabov V., Shurupov V., Suslova T., Markov V., Arterial Stiffness in Patients With Heart Failure And Preserved Ejection Fraction 12 Months After Myocardial Infarction. Polski Przeglad Kardiologiczny. 2012. 14 (3) P. 165-172.
2. Shah A.M., Claggett B., Desai A.S., Li G., Pfeffer M.A., Solomon S.D., Sweitzer N.K., Shah S.J., Anand I.S., O'Meara E., Rouleau J., Heitner J.F., Fang J., Zile M.R., Markov V., Ryabov V., Reis G., Assmann S.F., McKinlay S.M., Pitt B. et al. Cardiac Structure And Function And Prognosis In Heart Failure With Preserved Ejection Fraction. Circulation: Heart Failure. 2014. 7 (5) P. 740-751.
3. Ryabov VV, Kirgizova MA, Suslova TE, Karas SI, Markov VA, Karpov RS (2017). Relationships of growth factors, proinflammatory cytokines, and anti-inflammatory cytokines with long-term clinical results of autologous bone marrow mononuclear cell transplantation in STEMI. PLoS ONE 12(5): e0176900. https://doi.org/10.1371/journal.pone.0176900
4. Kercheva M., Ryabova T., Gusakova A., Suslova T.E., Ryabov V., Karpov R.S., Serum Soluble St2 and Adverse Left Ventricular Remodeling in Patients With St-Segment Elevation Myocardial Infarction. Clinical Medicine Insights: Cardiology. 2019. Vol. 13.
5. Gombozhapova A., Rogovskaya Y., Shurupov V., Rebenkova M., Popov S.V., Karpov R.S., Ryabov V., Kzhyshkowska J., Macrophage Activation and Polarization in Post-Infarction Cardiac Remodeling. Journal of Biomedical Science. 2017. Т. 24. No. 1. P. 1-11.
6. Ryabov V., Gombozhapova A., Rogovskaya Y., Rebenkova M., Karpov R., Kzhyshkowska J., Cardiac Cd68+ And Stabilin-1+ Macrophages in Wound Healing Following Myocardial Infarction: From Experiment to Clinic. Immunobiology. 2017. P. 413-421.

Keywords: Multiple Sclerosis, Neuroinflammation, Neurodegeneration, Biomarker, Diagnostic Panel, Excitotoxicity, Oxidative Stress, Demyelination

Research groups: Lean Implementation research, RUCTRIMS (Russian Committee for Treatment and Research in Multiple Sclerosis)

Research interests: Neuroimmunology, Neurodegeneration

Grants:

• Grant: Federal Target Program No. 14.607.21.0150 "Identification of biomarkers of the microbiotic intestinal community for early, preclinical diagnosis of Parkinson's disease"

Techniques, methods & infrastructure:

• Translational Research
• Clinical trials

Selected publications:

1.  Association of SNPs of CD40 Gene with Multiple Sclerosis in Russians/ M.A. Titova, V.M. Alifirova et al. //PLOS ONE, Vol. 8, No. 4, 2013, e61032 (www.plosone.org).
2. Influence of sleep disorders on the impulsive behavioral disorders among cognitively-intact Parkinson’s disease patients living in the Tomsk region, Russia / M. Titova, I. Zhukova, V. Alifirova et al.//European Journal of Neurology, 21 (Suppl. 1), 2014, 388–713, P.632.
3. Quality of life in patients receiving combination therapy with pramipexole / M. Titova, I. Zhukova, V. Alifirova et al.//European Journal of Neurology, 21 (Suppl. 1), 2014, 104–387, P.366
4.  Epidemiology of familial cases of multiple sclerosis in Tomsk region, Russian Federation / M. Titova, I. Zhukova, V. Alifirova et al.//European Journal of Neurology, Vol. 23, S 1, 2016, P. 412.
5. The effect of sleep and wakefulness disorders on cognitive function in patients with Parkinson’s disease/ M. Titova, I. Zhukova, V. Alifirova et al.//European Journal of Neurology, Vol. 23, S 1, 2016, P. 482.
6. The assessment of cognitive and mood disturbances in patients with Parkinson’s disease without dementia /M. Titova, I. Zhukova, V. Alifirova et al.//European Journal of Neurology, Vol. 23, S 1, 2016, P. 370.
7. TitovaM.A., Semkina A.A., Osmanova D.Z., Alifirova V.M., идр. Association of Polymorphic Variants of Brain-Derived Neurotrophic Factor Gene (Bdnf Rs6265) and Glutamate Transporter Gene of the Second Type (Slc1a2 Rs4354668) with the Course of Multiple Sclerosis in Patients Living in Tomsk Region // Annals of the Russian academy of medical sciences. - 2019. - Т. 74. – No. 1. - C. 14-19. doi: 10.15690/vramn1069

Keywords: Newborns, CNS Disorders, Neonatal Pathology, Children, Rehabilitation

Research interests: Clinical and metabolic disorders in newborns with different gestation periods

Techniques, methods and infrastructure:

• Clinical trials

Selected publications:

1. 1. Samoilova J.G., Sivolobova T.V., Kudlay D.A., Zhelev Y.A., Balanovsky A.P., Devald E.V., Rotkank M.A., Koshmeleva M.V., Petrnkovich A.M. Carbonhydrate metabolism disorfers in newborns with low and extremely low body weight. Pediatrics. 2020; 1:194-198. (in Russ)
2. Mikhalev E.V., Dmitrieva A.V., Loskova Elena Vladimirovna, Rafikova Y.S., Saprina TY., Kondratyeva E.I., Yankina G.N., Zhelev V.A., Krivonogova T.S., Ermolenko S.P., Golikova E.V. Peculiarities of nutritional status of children born with very low body weight. Pediatrics. 2018; 1(97):13-21. (in Russ)
3. Krivonogova T.S., Gerget O.M., Loshkova E.V. , Kondratyeva E.I., Zhelev V.A., Mikhalev E.V. , Barabash N.A., Golikova E.V., Dmitrieva A.V., Ermolenko S.P., Krivonogov N.N., Rafikova Y.S., Ryzhakova N. A., Terentyeva A.A., Tuteva E.Y, Shemyakina T.A., Yankina G.N. Antenatal prognosis of the outcomes of perinatal injury of the nervous system in children Questions of practical pediatrics. 2017; 6(12):7-15. (in Russ)
4. Gorlenko L.V., Yankina G.N., Davydova T.V, Vtorushin S.V., Loshkova E.V., Kondratyeva E.I., Zhelev V.A., Krivonogova T.S. Exudative enteropathy in a child of the first year of life 
5. Pediatrics. 2017; 6(96):176-182. (in Russ) 
6. Mihalev E.V., Balanovskiy A.P., Zhelev V.A., Ermolenko S.P., Solnishko A.L, Nagaeva T.A, Golikova E.V., Krivonogova T.S., Yankina G.N., Loshkova E.V. Eritrocytesˈ aggregative activity in newborns from mothers with different forms of fetoplacental insufficiency. Mother and child in Kuzbas. 2017; 4(71): 26-29. (in Russ

Keywords: Anemia of Chronic Disease, Iron Deficiency Anemia, Hyperlipidemia, Inflammation, C-reactive protein, Tumor Necrosis factor-α; Erythrocyte Sedimentation Rate 

Research groups: Personalized Medicine

Research interests: Molecular Medicine, Oncology, Hematology, Internal Medicine, Clinical Hematology, Hematologic Diseases, Hematopoiesis, Erythropoiesis

Selected publications:

1. Vasileva, O.A., Yakushina, V.D., Ryazantseva, N.V. et al. Regulation of gene expression of CD4+ T lymphocyte differentiation transcription factors by galectin-3 in vitro. Mol Biol 47, 879–884 (2013). https://doi.org/10.1134/S0026893313060150
2. Saprina, T. V., Zima, A. P., Musina, N. N., Prokhorenko, T. S., Latypova, A. V., Shakhmanova, N. S., и Budeeva, S. V. (2019). Pathogenetic aspects of hepcidin metabolism and ferrocinetics dysregulation in carbohydrate metabolism disorders. Diabetes Mellitus, 21(6), 506-512. doi: 10.14341/DM9378
3. N. Musina, N.; V. Saprina, T.; S. Prokhorenko, T.; Kanev, A.; P. Zima, A. Correlations between Iron Metabolism Parameters, Inflammatory Markers and Lipid Profile Indicators in Patients with Type 1 and Type 2 Diabetes Mellitus. J. Pers. Med. 2020, 10, 70.

Keywords: Iron Oxide Magnetic Nanoparticle, Nanoparticle Characterization, Tumor, Biodistribution, Cytotoxicity

Research groups: Population health under a global threat of trematode infection

Research interests: Molecular Biology, Microbiology, Biotechnology, Molecular cloning, Biochemistry, Nanoparticles, Nanobiotechnology, Magnetic Nanoparticles

Projects performed by the researcher:

1. Oncolytic activity of metal nano particles
2. Metabolic changes in the parasite-host interaction at the organ and organism level
3. Magnetic nanoparticles - DNA interactions
4. Chemical modification of the surface of magnetic nanoparticles by targeting peptides and quantum dots for biomedical application

Selected publications:

1. A.A. Nevmyvaka (A.A. Magaeva), A.G. Pershina, V.I. Itin. Nanosized magnetic powders based on oxides. Journal of Physics: Conference Series, Volume 1115, Issue 4, DOI: 10.1088/1742-6596/1115/4/042054
2. Saltykova I.V., Ittiprasert W, Nevskaya K.V., Dorofeeva Y.B., Kirillova N.A., Kulikov E.S., Ivanov V.V., Mann V.H., Pershina A.G. and Brindley P.J. (2019) Hemozoin From the Liver Fluke, Opisthorchis felineus, Modulates Dendritic Cell Responses in Bronchial Asthma Patients. Front. Vet. Sci. 6:332. doi: 10.3389/fvets.2019.00332
3. Alexandra G. Pershina, Olga Ya. Brikunova, Alexander M. Demin, Oleg B. Shevelev, Ivan A. Razumov, Evgenii L. Zavjalov, Dina Malkeyeva, Elena Kiseleva, Nadezhda V. Krakhmal’, Sergey V. Vtorushin, Vasily L. Yarnykh, Vladimir V. Ivanov, Raisa I. Pleshko, Victor P. Krasnov, Ludmila M. Ogorodova. pH-triggered delivery of magnetic nanoparticles depends on tumor volume, Nanomedicine: Nanotechnology, Biology and Medicine, Volume 23, 2020, 102086, ISSN 1549-9634, https://doi.org/10.1016/j.nano.2019.102086